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10.3390/ijms22052439 [DOI] [PMC free article] [PubMed] [Google Scholar] 13.Russo MV, McGavern DB

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The CL4176 worms that were synchronized to stage L4 and had not yet spawned were transferred to NGM culture plates with or without drug (0.5 mM), three plates per group, two worms per plate and placed at 16°C, which was recorded as the first day of worm reproduction, and thereafter the worms were transferred to a new plate every day until the worms no longer spawned