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Conversely, incorporating negatively charged lipids accelerates liposome clearance due to protein adsorption, while cationic lipids, typically used for nucleic acid delivery, can facilitate cellular uptake by condensing negatively charged nucleic acids [149]

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For LC-MS analysis, granulosa and cumulus cell extracts were resuspended in volumes proportional to their protein content (5 μL of 100% methanol was used per 5 μg/μL of protein content) with a minimum volume of 25 μL