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Frontiers | Natural flavonoids from herbs and nutraceuticals as ferroptosis inhibitors in central nervous system diseases: current preclinical evidence and future perspectives

250 mg aliquots of milled and dried sample were digested in 5 ml of trace analysis grade 65% nitric acid:perchloric acid (15:85, v/v) for 8.5 h at increasing temperatures [2 h at room temperature, 3 h at 60°C, 1 h at 100°C, 1 h at 120°C, 1.5 h at 175°C]

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Furthermore, metabolomic analysis from primary human pancreatic ductal adenocarcinoma (PDAC) tissue revealed a significant reduction of glutamine and several other NEAAs in the tumor tissues relative to the adjacent normal tissues as well (Kamphorst et al , 2015)