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To better understand the expression of MetE in dormant Mtb, we analyzed, by qRT-PCR, the transcription of metE gene at different stages of dormancy: in Figure 3B , we observed that while dormant bacilli showed the expected upregulation of the typical dormancy gene alpha-crystallin ( acr ) ( metE gene was clearly downregulated in 9- to 25-day-old hypoxic cultures
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