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Clinically, multi-parameter panels that integrate species-level reporters with damage footprints and iron metrics—interpreted with strict source attribution and compartment awareness—enable mechanism-anchored stratification and monitoring, preserving beneficial H 2 O 2 signalling while preventing convergence onto ONOO − , Fenton ·OH and runaway lipid peroxidation (Griendling et al
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Taken together, these studies offer insight into the diversity of functions that can occur outside of the active site in metabolic enzymes and build on recent discoveries of nonenzymatic “moonlighting” roles of metabolic enzymes
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