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For induction of lung tumor formation, 8–12-weeks-old mice were anesthetized with Ketavet (100 mg/kg) and Rompun (20 mg/kg) by intraperitoneal injection followed by intratracheal inhalation of replication-deficient adenovirus expressing Cre (Ad5-CMV-Cre, 2.5 × 10 7 PFU, University of Iowa) to their lungs 53 )

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Alcohol wipes: Used to sanitize the tops of the vials and maintain a sterile environment